Comparison of 624-type Capillary Columns

In our research, seven 624-type capillary columns were investigated. All the same in length, internal diameter, and film thickness (30 m × 0.32 mm 1.0 µm). However, they produced by different manufacturers or manufacturer but batches. Even though recommend them as "equivalent columns" this equivalence did not prevail even case of manufacturer. Our examination criteria centered on quantitative determination ability columns. A homemade column test mixture was compiled to represent all second-order interactions that can occur between analyte stationary phase. Although theoretically these have phase quality, result chromatograms. addition origin batch column, "history column" contributes likewise peak symmetry, retention order, areas affect determination. We quantified with effective carbon number (ECN) Limit Quantitation (LoQ) values. Based results attainable LoQ ECN values depend at least much actual state measurement conditions be optimized. paper, we demonstrate extent which phases offered companies and/or backgrounds influence detection limit detector response if relevant chemical structure.